Yuri N. Shkryl

Far Eastern Federal University.

Candidate of Science (03.00.23 – biotechnology, Institute of Biology and Soil Sciences, Far Eastern Branch of the Russian Academy of Sciences), associate professor

Level of English proficiency - В2-С1. 

Educational program and field of the educational program for which the applicant will be accepted

1.5.6 Biotechnology (biological sciences).

List of research projects of the potential supervisor (participation/leadership)

RFBR grant, 11-04-98556-r_vost_a, “Calcium-dependent protein kinases as a new tool for plant metabolic engineering”, supervisor.

Grant of the Far Eastern Branch of the Russian Academy of Sciences, 12-III-A-06-080, “Study of the molecular mechanisms of regulation of anthraquinone biosynthesis in transgenic crops of madder Rubia cordifolia L.”, supervisor.

Grant of the Far Eastern Branch of the Russian Academy of Sciences, 15-I-6-102, “Study of the cytotoxic effect of metal nanoparticles in a cell system in vitro”, supervisor.

Grant of the Far Eastern Branch of the Russian Academy of Sciences, 18-3-045, "Development and research of biomimetic principles for the creation of semiconductor and genetically encoded biosensors", supervisor.

RFBR grant, 19-34-90184 “Preparation and properties of metal nanoparticles in an in vitro cell system”, supervisor.

RSF grant, 22-24-00082 "Functional properties and biological role of the rolB/C oncogene from the natural transgenic agricultural plant sweet potato", supervisor.

Project 6N of the Advanced Engineering School "Institute of Biotechnology, Bioengineering and Food Systems" FEFU, supervisor.

List of the topics offered for the prospective scientific research

Heterologous expression of recombinant proteins in plants and microorganisms;

Regulation of vitamin biosynthesis by microbial producers;

Creation of recombinant vaccines;

Regulation of biosynthesis of low molecular weight compounds in plants.

Supervisor’s research interests:

Molecular biology and biotechnology; genetic and genomic engineering of plants, fungi and microorganisms; biomimetics, organic and inorganic nanostructures; drug compounds, vaccines and therapeutic proteins.

Research highlights

The opportunity to conduct joint research with the industrial partner of the FEFU NSP - the Arnika company.

Supervisor’s specific requirements:

High level of knowledge of basic biological disciplines: molecular biology, recombinant DNA technology, microbiology, etc.

Basic knowledge of laboratory methods: microscopy, nucleic acid extraction, PCR.

Ability to carry out statistical processing of research results.

The level of English is sufficient for fluent communication and writing.

Supervisor’s publications

Over the past 5 years, 30 papers have been published, including 25 scientific articles in journals indexed by Web of Science (of which 15 are Q1), 4 articles in Scopus and 1 article in an RSCI-listed journal.

Supervisor’s main publications:

1. Shkryl Y., Yugay Y., Avramenko T., Grigorchuk V., Gorpenchenko T., Grischenko O., Bulgakov V. (2021) CRISPR/Cas9-mediated knockout of HOS1 reveals its role in the regulation of secondary metabolism in Arabidopsis thaliana. Plants (Basel) 10:104. DOI: 10.3390/plants10010104.

2. Shkryl Y., Rusapetova T., Yugay Y., Egorova A., Silant’ev V., Grigorchuk V., Karabtsov A., Timofeeva Y., Vasyutkina E., Kudinova O., Ivanov V., Kumeiko V., Bulgakov V. (2021) Biosynthesis and cytotoxic properties of Ag, Au, and bimetallic nanoparticles synthesized using Lithospermum erythrorhizon callus culture extract // International Journal of Molecular Sciences. V.22, P.9305; DOI: 10.3390/ijms22179305.

3. Shkryl Y., Veremeichik G., Avramenko T., Gorpenchenko T., Tchernoded G., Bulgakov V. (2022) Transcriptional regulation of enzymes involved in ROS metabolism and abiotic stress resistance in rolC-transformed cell cultures. Plant Growth Regulation 97, 485-497. DOI: 10.1007/s10725-022-00812-1.

4. Shkryl Y., Yugay Y., Vasyutkina E., Chukhlomina E., Rusapetova T., Bulgakov V. (2022) The RolB/RolC homolog from sweet potato promotes early flowering and triggers premature leaf senescence in transgenic Arabidopsis thaliana plants // Plant Physiology and Biochemistry 193, 50–60. DOI: 10.1016/j.plaphy.2022.10.018.

5. Shkryl Y.N., Tchernoded G.K., Yugay Y.A., Grigorchuk V.P., Sorokina M.R., Gorpenchenko T.Y., Kudinova O.D., Degtyarenko A.I., Onishchenko M.S., Shved N.A., Kumeiko V.V., Bulgakov V.P. 2023. Enhanced Production of Nitrogenated Metabolites with Anticancer Potential in Aristolochia manshuriensis Hairy Root Cultures. International Journal of Molecular Sciences 24:11240. https://doi.org/10.3390/ijms241411240.

Results of intellectual activity

1. RF Patent No. 0002679390, 02/07/2019. Recombinant plasmid DNA pSAT1-AhA1, encoding storage protein A1 of Amaranthus hypochondriacus seeds, and recombinant strain Myceliophthora thermophila/pSAT1-AhA1 - producer of storage protein A1 // Balabanova L.A., Shkryl Yu.N., Slepchenko L.V., Yugay Yu.A., Podvolotskaya A.B., Son O.M., Tekutyeva L.A.

2. RF Patent No. 0002680295, 02/19/2019. Recombinant plasmid DNA psat1-zmzeinb1, encoding the feed protein alpha-zein B1 of corn species zea mays, and the recombinant strain myceliophthora thermophila/psat1-zmzeinb1 - producer of the feed protein alpha-zein B1 // Tekutyeva L.A., Balabanova L.A., Son O.M., Podvolotskaya A.B., Marchenok M.V., Yugay Yu.A., Slepchenko L.V., Shkryl Yu.N.

3. RF Patent No. RU 2756113 C1, 02.09.2020. Recombinant plasmid DNA pMF230-GSMT/DMT, encoding a chimeric enzyme for betaine biosynthesis, and a recombinant strain of Pseudomonas denitrificans/pMF230-GSMT/DMT - a betaine producer // Shkryl Yu.N., Balabanova L.A., Degtyarenko A.I., Grigorchuk V.P., Slepchenko L.V., Marchenok M.V., Podvolotskaya A.B., Son O.M., Tekutyeva L.A.